Device

Part:BBa_K4062028:Design

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-16)


lactose regulated bla, RBS mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2919
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1987


Design Notes

The RBS for the gene downstream of the lacI biobrick is inactivated. No expression of this gene is expected.


Source

Sequence from the Escherichia coli K12 genomic library from biocyc.org

References